Using computations to analyze and reshape metabolism for bioproduction
| Speaker:
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Dr. Costas D. Maranas
Waltemeyer Endowed Chair in Chemical Engineering Department of Chemical Engineering The Pennsylvania State University, USA
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| Theme: | Using computations to analyze and reshape metabolism for bioproduction |
| Abstract: | Metabolism is defined as the full complement of chemical transformations in living systems. Our group has previously introduced a variety of computational tools (i.e., OptKnock, OptForce) for shaping host native metabolism towards various overproduction goals. However, in many cases the native metabolic repertoire needs to be expanded upon. To support this goal, we will discuss computational tools for constructing thermodynamically feasible, carbon and energy efficient synthesis pathways. Protein engineering, either through de novo design or directed evolution, can expand upon the parts-list available for constructing pathways by re-designing existing enzymes to carry out novel conversions. Leveraging these capabilities, we have developed retrosynthesis tools which allow for seamlessly blending known biochemical
reactions with de novo steps to construct atom and energy balanced synthesis pathways. Machine learning methods will be introduced for predicting enzyme kinetics coupled with the deployment of kinetic models with a genome-wide coverage for assessing the efficacy of identified designs. Insight and lessons gained from the application of these tools on a variety of bioproduction challenges as members of the bioenergy centers CBI and CABBI as well as the NSF AI retrosynthesis institute MMLI will be provided. |
| Short CV: | https://www.maranasgroup.com/people.htm |
| Language | English
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