Multi-scale Media Optimization for 3-Hydroxypropionic Acid Production in Rhodosporidium toruloides
| Speaker: | Dr. Apostolos Zournas
Quantitative Metabolic Modeling Group, Joint Bioenergy Institute (JBEI) Lawrence Berkeley National Laboratory (LBNL), USA |
| Theme: | Multi-scale Media Optimization for 3-Hydroxypropionic Acid Production in Rhodosporidium toruloides |
| Abstract: | The successful translation of synthetic biology innovations into commercially viable biomanufacturing processes is often hindered by challenges associated with process scale-up. This study presents a systematic methodology that employs artificial intelligence to streamline these efforts. We demonstrate the application of active learning for the design of optimized culture media for 3-hydroxypropionic acid (3-HP) production in Rhodosporidium toruloides at a microplate scale (0.8 mL). Furthermore, we illustrate how these micro-scale data, when combined with a limited dataset from a larger bench-scale (150 mL), can be used to accurately predict 3-HP titers across divergent scales. This work highlights the potential of transfer learning to address key challenges in bioprocess scale-up, thereby improving product titers and yields and contributing to the development of a sustainable and profitable bioeconomy. |
| Short CV: | Education
Doctor of Philosophy (PhD), Chemical and Biochemical Engineering • Institution: Rutgers, The State University of New Jersey • Dissertation: “Computational and Biophysical Tools to Investigate the Coupling of the Light and Dark Reactions of Photosynthetic Organisms at the Photosystem and Cellular Levels” • Advisor: Gerard C. Dismukes Master of Science (MS), Chemical and Biochemical Engineering • Institution: Rutgers, The State University of New Jersey • Thesis: “INST-FLUX: An Open-Source Tool for Isotopically Non-Stationary Metabolic Flux Analysis (INST-MFA)” • Advisor: Desmond Lun Master of Science (MS), Horticultural Genetics and Biotechnology • Institution: Mediterranean Agronomic Institute of Chania (MAICH) • Thesis: “Flux Balance Analysis for the Tomato Plant (Solanum lycopersicum)” • Advisor: Maria Klapa, FORTH-ICE/HT, MESBL Diploma of Engineering (Dipl. Eng.), Environmental Engineering • Institution: Technical University of Crete, Chania, Greece (2014) • Thesis: “Intergrated nitrification denitrification using encapsulated microorganisms” • Advisor: Petros Gikas
Postdoctoral Research Fellow • Group: Quantitative Metabolic Modeling Group, Joint Bioenergy Institute (JBEI), Lawrence Berkeley National Laboratory (LBNL) • Field of Research: Applications of machine learning in synthetic biology and biomanufacturing. • Advisor: Hector Garcia-Martin |
| Language: | English – Greek
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